HPLC Analysis of Moxifloxacin in Rabbit Aqueous Humor after Topical Instillation of Mucoadhesive Hyaluronate Formulations

Here we provide an extremely simple, rapid, direct, sensitive and specific HPLC method to quantify moxifloxacin in rabbit aqueous humor. Separation of moxifloxacin was performed on a Kromasil C8 column using a ternary pre-mixed mobile phase of acetonitrile, methanol and 0.02 M KH2PO4 buffer solution containing 1% triethylamine (pH 3.0 with phosphoric acid) (15:20:65, v/v/v). UV detection at 292 nm was used with a flow rate of 1 ml/min. The developed method fulfills the validation requirements of FDA. Consequently, it is suitable to investigate the pharmacokinetic profile of ophthalmic formulation based on moxifloxacin. As an example, we investigated the pharmacokinetic profile of two new ophthalmic moxifloxacin mucoadhesive formulations based on sodium hyaluronate. The pharmacokinetic profiles outlined showed a strong bioavailability increase for moxifloxacin-hyaluronate based formulation with respect to the marketed conventional eye drop.